Urinary S-phenylmercapturic Acid as a Key Biomarker for Measuring Occupational Exposure to Low Concentrations of Benzene in Chinese Workers A Pilot Study

作者：Lv, Bi-hua; Song, Shi-zhen; Zhang, Zhibing; Mei, Yong; Ye, Fang-li^*

来源：Journal of Occupational and Environmental Medicine, 2014, 56(3): 319-325.

DOI：10.1097/JOM.0000000000000098

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• TRANS-MUCONIC ACID
• TRANS,TRANS-MUCONIC ACID
• PERSONAL EXPOSURE
• METABOLITES
• VALIDATION
• CHILDHOOD
• TOLUENE

Objective: This study analyzed the level of urinary S-phenylmercapturic acid (U-SPMA) for low benzene exposure in a group of Chinese shoe-making workers.
Methods: Urinary samples from 55 workers exposed to benzene at levels lower than 10 parts per million (ppm) were collected at postshift. U-SPMA level was determined using high-performance liquid chromatography/mass spectrography (HPLC/MS) method.
Results: Good linearity of U-SPMA was observed within the range from 10 to 320 g/L (r = 0.9994). Concentration of airborne benzene ranged from 0.71 to 32.17 mg/m(3), and three segments were divided with different levels of exposure (6.0, 6.0 to 10.0, 10 to 32.5 mg/m(3)), the median U-SPMA concentrations were 49.55, 102.15, and 335.69 g/g Cr, respectively.
Conclusion: A good linear correlation was found between U-SPMA levels and airborne benzene concentrations. The selected method could be applied for detecting other working conditions in China.

• 出版日期2014-3
• 单位武汉科技大学; 武汉大学