Anti-inflammatory effects of physalin E from Physalis angulata on lipopolysaccharide-stimulated RAW 264.7 cells through inhibition of NF-κB pathway

作者：Yang, Yan-Jun^*; Yi, Lang; Wang, Qing; Xie, Bing-Bing; Dong, Yan^*; Sha, Cong-Wei

来源：Immunopharmacology and Immunotoxicology, 2017, 39(2): 74-79.

DOI：10.1080/08923973.2017.1282514

• Physalin E
• cytokine
• nuclear factor-kappa B
• glucocorticoid receptor
• lipopolysaccharide
• macrophage

Physalin E is a naturally occurring seco-steroid isolated from the stems and aerial parts of Physalis angulata L. (Solanaceae). This study was aimed to explore the anti-inflammatory effects of physalin E on RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS) and the potential underlying mechanisms. The results showed that physalin E significantly inhibited LPS-induced tumor necrosis factor- (TNF-) and interleukin-6 (IL-6) expression and secretion in a dose-dependent manner. Unlike dexamethasone, these effects could not be blocked by miferstone (RU486). Meanwhile, physalin E reduced the degradation of I-kappa B protein in the cytoplasm and downregulated the nuclear factor-B (NF-B) p65 protein in the nuclear, which resulted in the inhibition of the NF-B nuclear translocation. In conclusion, physalin E exerts its anti-inflammatory activities in LPS-induced macrophages. Physalin E can inhibit the production of inflammatory cytokines by targeting the NF-B signaling pathway.

• 出版日期2017
• 单位广州中医药大学; 广东食品药品职业学院