A physical map of the Mycoplasma agalactiae strain PG2 genome

作者：Tola S^*; Idini G; Rocchigiani AM; Rocca S; Manunta D; Leori G

来源：Veterinary Microbiology, 2001, 80(2): 121-130.

DOI：10.1016/S0378-1135(00)00389-8

• Mycoplasma agalactiae
• physical map
• pulsed field gel electrophoresis

We have constructed a physical map of the Mycoplasma agalactiae strain PG2 chromosome analyzing it by pulsed field gel electrophoresis in a contour-clamped homogeneous electric-field system. We mapped 33 cleavage sites generated with SmaI, XhoI, SalI, EclXI and BsiWI restriction endonucleases using double digestions, one- and two-dimensional pulsed electrophoresis, crosshybridization and linking clones. We have also mapped the loci of some genes by Southern hybridization.

• 出版日期2001-5-21