Apo lipoprotein E isoforms disrupt long-chain fatty acid distribution in the plasma, the liver and the adipose tissue of mice

作者：Conway Valerie; Larouche Annie; Alata Wael; Vandal Milene; Calon Frederic; Plourde Melanie^*

来源：Prostaglandins, Leukotrienes and Essential Fatty Acids, 2014, 91(6): 261-267.

DOI：10.1016/j.plefa.2014.09.007

• Transport and binding proteins
• n-3 PUFA metabolism
• Cellular FA uptake and degradation
• APOE4 carriers

Evidences suggest that omega-3 fatty acid (n-3 PUFA) metabolism is imbalanced in apolipoprotein E epsilon 4 isoform carriers (APOE4). This study aimed to investigate APOE genotype-dependant modulation of FA profiles, protein and enzyme important to fatty acid (FA) metabolism in the adipose tissue, the liver and the plasma using human APOE-targeted replacement mouse-model (N=37). FA transport (FATP) and binding (FABP) protein levels in tissues and concentrations of liver camitine palmitoyltransferase 1 (CPT1) were performed. N-3 PUFA concentration was %26gt; 45% lower in the adipose tissue and liver of APOE4 mice compared to APOE3 mice. In APOE4 mice, there were higher levels of FATP and FABP in the liver and higher FATP in the adipose tissue compared to APOE2 mice. There was a trend towards higher CPT1 concentrations in APOE4 mice compared to APOE3 mice. Therefore, since APOE-isoform differences were not always in line with the unbalanced n-3 PUFA profiles in organs, other proteins may be involved in maintaining n-3 PUFA homeostasis in mice with different APOE-isoforms.

• 出版日期2014-12