Atrazine induced oxidative stress and mitochondrial dysfunction in quail (Coturnix C-coturnix) kidney via modulating Nrf2 signaling pathway

作者：Zhang, Cong; Qin, Lei; Dou, Da-Chang; Li, Xue-Nan; Ge, Jing; Li, Jin-Long^*

来源：Chemosphere, 2018, 212: 974-982.

DOI：10.1016/j.chemosphere.2018.08.138

• Atrazine
• Quail
• Nephrotoxicity
• Mitochondrial dysfunction
• Oxidative stress
• Nrf2 signaling pathway

Atrazine (AIR) is a most used herbicide which is believed as a pivotal determinant of environmental nephrosis, but potential mechanism is still largely unclear. This study intends to reveal a novel mechanism of AIR-induced nephrotoxicity. Quail were treated with 0, 50, 250 and 500 mg AIR/kg/d by oral gavage for 45 days. Kidney coefficient was decreased, biochemical and morphologic indices reflecting the kidney injury were significantly increased in ATR-exposed quail. AIR exposure upregulated the expression of proapoptotic factors (Bax, Caspase 3 and FasL) and downregulated antiapoptotic factor (Bcl-2). Notably, cristae of mitochondria decreased, mitochondrial malformation and mitochondrial vacuolar degeneration were observed in ATR-exposed quail. AIR induced the disorder of mitochondrial function related factors expressions and promoted oxidative damage. Furthermore, ATR induced toxicities in the expression of Nrf2 and Nrf2-target genes. In conclusion, AIR altered the microstructure and function of quail kidney. ATR induced renal damage via causing mitochondrial dysfunction, influencing mitochondrial function related genes expression, modulating Nrf2 signaling pathway. This study suggested ATR induced the nephrotoxicity via disturbing the transcription of mitochondrial function related factors and Nrf2 signaling pathway.

• 出版日期2018-12
• 单位齐齐哈尔医学院; 东北农业大学