Abl deregulates Cdk5 kinase activity and subcellular localization in Drosophila neurodegeneration

作者：Lin H; Lin T Y; Juang J L^*

来源：Cell Death and Differentiation, 2007, 14(3): 607-615.

DOI：10.1038/sj.cdd.4402033

• Abl
• Cdk5
• Drosophila
• Alzheimer's disease
• neurodegeneration

Although Abl functions in mature neurons, work to date has not addressed Abl's role on Cdk5 in neurodegeneration. We found that beta-amyloid (A beta 42) initiated Abl kinase activity and that blockade of Abl kinase rescued both Drosophila and mammalian neuronal cells from cell death. We also found activated Abl kinase to be necessary for the binding, activation, and translocalization of Cdk5 in Drosophila neuronal cells. Conversion of p35 into p25 was not observed in A beta 42-triggered Drosophila neurodegeneration, suggesting that Cdk5 activation and protein translocalization can be p25-independent. Our genetic studies also showed that abl mutations repressed A beta 42-induced Cdk5 activity and neurodegeneration in Drosophila eyes. Although A beta 42 induced conversion of p35 to p25 in mammalian cells, it did not sufficiently induce Cdk5 activation when c-Abl kinase activity was suppressed. Therefore, we propose that Abl and p35/p25 cooperate in promoting Cdk5-pY15, which deregulates Cdk5 activity and subcellular localization in Ab42-triggered neurodegeneration.

• 出版日期2007-3