Adoptive transfer of transforming growth factor-1-induced CD4(+)CD25(+) regulatory T cells prevents immune response-mediated spontaneous abortion

作者：Qiu, Tian; Teng, Yincheng; Wang, Yudong; Xu, Liang^*

来源：Reproduction, Fertility and Development, 2016, 28(11): 1788-1797.

DOI：10.1071/RD14503

• forkhead box P3 (FOXP3)

The effects of adoptive transfer of transforming growth factor (TGF)-1-induced regulatory T (T-reg) cells in preventing spontaneous abortion in mice were investigated. CD4(+)CD25(-) cells were isolated from the spleens of pregnant CBA/J mice and induced into T-reg cells positive for CD4, CD25 and forkhead box P3 (FOXP3) ex vivo using interleukin (IL)-2 and TGF-1. CBA/J mice were mated with DBA/2J mice to establish a model of spontaneous abortion and, on the first day of pregnancy, mice were injected intravenously with 2x10(5) either freshly isolated T-reg cells or those induced with TGF-1. After 14 days, the surviving and reabsorbed fetuses in both groups were counted, and serum cytokine concentrations were measured by ELISA. Adoptive transfer of CD4(+)CD25(+) or TGF-1-induced T-reg cells significantly reduced the fetal resorption rate, increased serum IL-10 and TGF-1 concentrations and decreased interferon- levels. In conclusion, the results of the present study indicate that adoptive transfer of TGF-1-induced T-reg cells prevents spontaneous abortion in mice by increasing the secretion of T helper (Th) 2 cytokines and decreasing the secretion of Th1 cytokines.

• 出版日期2016
• 单位上海交通大学