Application of Recombinant Gnathostoma spinigerum Matrix Metalloproteinase-Like Protein for Serodiagnosis of Human Gnathostomiasis by Immunoblotting

作者：Janwan Penchom; Intapan Pewpan M; Yamasaki Hiroshi; Laummaunwai Porntip; Sawanyawisuth Kittisak; Wongkham Chaisiri; Tayapiwatana Chatchai; Kitkhuandee Amnat; Lulitanond Viraphong; Nawa Yukifumi; Maleewong Wanchai^*

来源：The American Journal of Tropical Medicine and Hygiene, 2013, 89(1): 63-67.

DOI：10.4269/ajtmh.12-0617

Matrix metalloproteinase (MMPs) is the extracellular zinc-dependent endopeptidase and is secreted for degrading extracellular matrix molecules of host tissues. A cDNA encoding MMP-like protein of Gnathostoma spinigerum larvae was amplified by reverse transcription-polymerase chain reaction, and was cloned into a prokaryotic expression vector, and expressed in Escherichia coli. Total immunoglobulin G class (total IgG) antibody responses to the recombinant MMP-like protein were analyzed by immunoblot diagnosis of human gnathostomiasis. Serum samples from proven and clinically suspected cases of gnathostomiasis, other parasitic diseases patients, and from healthy volunteers were tested. The immunoblotting gave high sensitivity (100%) and specificity (94.7%). Positive and negative predictive values were 85.4% and 100%, respectively. Recombinant MMP-like protein can be used as a diagnostic antigen and potentially replace native parasite antigens to develop a gnathostomiasis diagnostic kit.

• 出版日期2013-7