Natural and emergent Trypanosoma cruzi I genotypes revealed by mitochondrial (Cytb) and nuclear (SSU rDNA) genetic markers

作者：David Ramirez Juan; Clara Duque Maria; Montilla Marleny; Cucunuba Zulma; Guhl Felipe

来源：Experimental Parasitology, 2012, 132(4): 487-494.

DOI：10.1016/j.exppara.2012.09.017

• DTU's
• Recombination
• Genotypes
• Cytochrome b
• Phylogenetic networks
• Maximum likelihood

Chagas disease is a tropical and systemic disease caused by the parasite Trypanosoma cruzi. This parasite has been divided into six Discrete Typing Units (DTU's) due to its high genetic diversity. T. cruzi I (RI) is the most prevalent DTU in Colombia and recently associated to cardiomyopathies. The aim of this study was to unravel the genetic variability among a set of 70 cell-single Tcl clones from different geographical regions and hosts using the sequences of Cytb and SSU rDNA. The results showed two genotypes associated to transmission cycles of Chagas disease in Colombia and supports the previous descriptions using SL-IR. Phylogenetic networks were developed detecting recombination events within Tcl. We also tested the phylogenetic relationships beneath Tcl clones and TcIII/TcIV sequences observing the high relatedness of Tcl clones from sylvatic cycle with TcIII/TcIV. We corroborate the high genetic diversity displayed by Tcl, the plausible recombination within this DTU supporting the previous model of genetic exchange proposed in T. cruzi populations. We conclude inquiring the need to pursue new studies to elucidate the genetic structure of Tcl across Chagas disease endemic countries.

• 出版日期2012-12