Molecular Background of Estrogen Receptor Gene Expression in Endometriotic Cells

作者：Izawa Masao^*; Taniguchi Fuminori; Harada Tasuku

来源：Reproductive Sciences, 2016, 23(7): 871-876.

DOI：10.1177/1933719115623642

• estrogen receptor
• estrogen receptor
• mRNA expression
• endometriosis

The molecular background of estrogen receptor (ER) expression is important to understand the pathophysiology of the high estrogen environment in endometriosis. However, the molecular details have not been fully understood. The objective of this study is to evaluate the molecular background of ER and ER messenger RNA (mRNA) expression in endometriotic cells. The following summarizes our observations: (1) ER mRNA expression in endometriotic cells was estimated to be approximately one-tenth of that in endometrial cells. (2) Three mRNAs, which include 3 different 5-untranslated exons tagged to an open reading frame of wild-type ER, were detected. (3) Expression of ER mRNA depends mostly on 0N promoter and includes 2 open reading frames: one for a wild-type ER1 and another for a splice variant ER2. (4) Expression of ER1 mRNA was approximately 40-fold higher than that in endometrial cells. (5) Expression of ER2 mRNA was almost at a comparable level of the ER1. 9 (6) ER and ER mRNAs are equivalently expressed in endometriotic cells. These observations show the molecular background of ER mRNA expression in endometriotic cells and provide a clue to further understanding the estrogen-dependent pathophysiology leading to clinical application in endometriosis.

• 出版日期2016-7