Stem cell adhesion and proliferation on hydrolyzed poly(butylene succinate)/beta-tricalcium phosphate composites

作者：Pa***irapong Somying; Singhatanadgit Weerachai; Meesap Preeyapan; Theerathanagorn Tharinee; Toso Montree; Janvikul Wanida^*

来源：Journal of Biomedical Materials Research Part A, 2015, 103(2): 658-670.

DOI：10.1002/jbm.a.35214

• surface hydrolysis
• poly(butylene succinate)
• calcium phosphate
• cell adhesion
• cell proliferation

Although poly(butylene succinate)/beta-tricalcium phosphate (PBSu/TCP) composites are biocompatible and allow the growth and osteogenic differentiation of stem cells, cell attachment and adhesion to the PBSu-based substrates is often limited. To enhance cell adhesion and proliferation, we used a sodium hydroxide (NaOH) hydrolysis technique to generate a different degree of roughness on PBSu/TCP substrates with different PBSu: TCP ratios. The results showed that NaOH hydrolysis increased surface roughness of PBSu/TCP substrates in a concentration-dependent manner. Substrates with higher ratios of TCP: PBSu provided more porous topography after NaOH hydrolysis, with a substrate containing 40 wt % TCP (PBSu/TCP-6040) hydrolyzed with 1.5M NaOH (HPBSu/TCP-6040-1.5) showing the highest degree of roughness. As with the roughness, PBSu/TCP surface hydrophilicity was positively affected by the increasing NaOH concentration and TCP incorporation. Stem cells adhered best on HPBSu/TCP-6040-1.5 with three-dimensionally elongated cell extensions. Moreover, the HPBSu/TCP-6040-1.5 substrate most significantly facilitated stem cell actin cytoskeleton reorganization and vinculin-positive focal adhesion formation when compared with the other substrates tested. HPBSu/TCP-6040-1.5 also demonstrated the greatest increase in cell proliferation when compared with the other substrates studied. In conclusion, the results have shown that among various substrates tested, HPBSu/TCP-6040-1.5 provided the best support for stem cell adhesion and proliferation, suggesting its potential use in bone engineering.

• 出版日期2015-2