An intermediate-conductance Ca2+-activated K+ channel is important for secretion in pancreatic duct cells

作者：Hayashi Mikio^*; Wang Jing; Hede Susanne E; Novak Ivana

来源：American Journal of Physiology - Cell Physiology, 2012, 303(2): C151-C159.

DOI：10.1152/ajpcell.00089.2012

• HCO3- transport
• H+-K+ pump
• K+ secretion
• K(ca)3.1
• KCNQ1

Hayashi M, Wang J, Hede SE, Novak I. An intermediate-conductance Ca2+-activated K+ channel is important for secretion in pancreatic duct cells. Am J Physiol Cell Physiol 303: C151-C159, 2012. First published May 2, 2012; doi:10.1152/ajpcell.00089.2012.Potassium channels play a vital role in maintaining the membrane potential and the driving force for anion secretion in epithelia. In pancreatic ducts, which secrete bicarbonate-rich fluid, the identity of K+ channels has not been extensively investigated. In this study, we investigated the molecular basis of functional K+ channels in rodent and human pancreatic ducts (Capan-1, PANC-1, and CFPAC-1) using molecular and electrophysiological techniques. RT-PCR analysis revealed mRNAs for KCNQ1, KCNH2, KCNH5, KCNT1, and KCNT2, as well as KCNN4 coding for the following channels: KVLQT1; HERG; EAG2; Slack; Slick; and an intermediate-conductance Ca2+-activated K+ (IK) channel (KCa3.1). The following functional studies were focused on the IK channel. 5,6-Dichloro-1-ethyl-1,3-dihydro2H- benzimidazole-2-one (DC-EBIO), an activator of IK channel, increased equivalent short-circuit current (I-sc) in Capan-1 monolayer, consistent with a secretory response. Clotrimazole, a blocker of IK channel, inhibited I-sc. IK channel blockers depolarized the membrane potential of cells in microperfused ducts dissected from rodent pancreas. Cell-attached patch-clamp single-channel recordings revealed IK channels with an average conductance of 80 pS in freshly isolated rodent duct cells. These results indicated that the IK channels may, at least in part, be involved in setting the resting membrane potential. Furthermore, the IK channels are involved in anion and potassium transport in stimulated pancreatic ducts.

• 出版日期2012-7