Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis

作者:Cangelosi Gerard A; Weigel Kris M; Lefthand Begay Clarita; Meschke John S*
来源:Applied and Environmental Microbiology, 2010, 76(3): 960-962.
DOI:10.1128/AEM.01810-09

摘要

Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

  • 出版日期2010-2