科研之友
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摘要
In order to determine the active ingredients in root extracts of Panax quinquefolius (American ginseng), a gradient HPLC method involving UV photodiode array detection was applied to separate and quantify simultaneously the ginsenosides Rb-1, Rb-2, Rc, Rd, Re, Rf and Rg(1). All ginseng saponins were baseline-resolved under the selected conditions, and the detection limits were 1.0 mu g/mL or less. The method has been applied to analyse ginsenosides extracted from American ginseng cultivated in both Wisconsin and Illinois. Ginsenosides Re and Rb-1 were the two main ginseng saponins in the root. The amounts of Re in 5- and 7-year Illinois-cultivated samples were greater than those found in ginseng cultivated for 3 or 4 years in Wisconsin, whereas the levels of Rb-1 were greater in the younger Wisconsin samples.
- 出版日期2005-8
