Simultaneous determination of nine intense sweeteners in foodstuffs by high performance liquid chromatography and evaporative light scattering detection - Development and single-laboratory validation

作者：Wasik Andrzej; McCourt Josephine; Buchgraber Manuela^*

来源：Journal of Chromatography A, 2007, 1157(1-2): 187-196.

DOI：10.1016/j.chroma.2007.04.068

• single-laboratory validation
• HPLC-ELSD
• multiple sweeteners
• foodstuffs

A high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) has been developed for the simultaneous determination of multiple sweeteners, i.e., acesulfame-K, alitame, aspartame, cyclamic acid, dulcin, neotame, neohesperidine dihydrochalcone, saccharin and sucralose in carbonated and non-carbonated soft drinks, canned or bottled fruits and yoghurt. The procedure involves an extraction of the nine sweeteners with a buffer solution, sample clean-up using solid-phase extraction cartridges followed by an HPLC-ELSD analysis. The trueness of the method was satisfactory with recoveries ranging from 93 to 109% for concentration levels around the maximum usable dosages for authorised sweeteners and from 100 to 112% for unauthorised compounds at concentration levels close to the limit of quantification (LOQs). Precision measures showed mean repeatability values of <4% (expressed as relative standard deviation) for highly concentrated samples and <5% at concentration levels close to the LOQs. Intermediate precision was in most cases <8%. The limits of detection (LODs) were below 15 mu g g(-1) and the LOQs below 30 mu g g(-1) in all three matrices. Only dulcin showed slightly higher values, i.e., LODs around 30 mu g g(-1) and LOQs around 50 mu g g(-1).

• 出版日期2007-7-20