Post-synthetic Modification of 3 ' Terminus of RNA with Propargylamine: A Versatile Scaffold for RNA Labeling through Copper-catalyzed Azide-Alkyne Cycloaddition

作者：Yamamoto Junpei^*; Ebisuda Shohei; Kong Lingqi; Yamago Haruka; Iwai Shigenori

来源：Chemistry Letters, 2017, 46(5): 767-770.

DOI：10.1246/cl.170158

• RNA labeling
• Alkynylation
• CuAAC reaction

We report the chemical alkynylation of oligoribonucleotides with propargylamine. Stepwise treatments of oligoribonucleotides with NaIO4, propargylamine, and NaBH3CN yielded N-propynylmorpholine derivatives at the 3' terminus. This RNA labeling approach is applicable to any natural RNA that possesses a ribonucleoside at the 3' terminus.

• 出版日期2017-5