A novel and hybrid composite transposon at the origin of acquisition of bla(RTG-5) in Acinetobacter baumannii

作者：Bonnin Remy A; Poirel Laurent; Nordmann Patrice^*

来源：International Journal of Antimicrobial Agents, 2012, 40(3): 257-259.

DOI：10.1016/j.ijantimicag.2012.04.002

• beta-Lactamase
• Insertion sequence
• Genetic context
• Transposon

The genetic context of the gene coding for the novel variant of the class A beta-lactamase RTG-5 (CARB-14), exhibiting two amino acid substitutions compared with the closest RTG-3 enzyme, was analysed in a clinical isolate of Acinetobacter baumannii. This novel variant exhibited a narrow-spectrum profile, as observed for RTG-3. Sequence analysis of the region flanking the 5' end of bla(CARB-14) identified a novel insertion sequence ISAba21 belonging to the IS3 family. Downstream of bla(CARB-14), another insertion sequence belonging to the IS3 family named ISAba14 was identified. Analysis of the left-end extremity of ISAba21 and the right-end extremity of ISAba14 identified direct repeat sequences of 3 bp, likely being the signature of a transposition process. This is one of the few examples of a composite transposon made of different insertion sequences bracketing a gene coding for this novel carbenicillinase.

• 出版日期2012-9