Antioxidant/antihyperglycemic activity of phenolics from sugarcane (Saccharum officinarum L.) bagasse and identification by UHPLC-HR-TOFMS

作者：Zheng, Rui; Su, Shan; Zhou, Huifang; Yan, Huaifeng; Ye, Junhua; Zhao, Zhengang^*; You, Lijun; Fu, Xiong^*

来源：Industrial Crops and Products, 2017, 101: 104-114.

DOI：10.1016/j.indcrop.2017.03.012

• Sugarcane bagasse
• Phenolics
• Antioxidant
• Antidiabetes
• UHPLC-HR-TOFMS

Sugarcane bagasse, one of the most abundant agro-food by-products, is a very promising raw material available at low cost for recovering bioactive substances. In this study, the total phenolic content was measured by Folin-Ciocalteu method and the antioxidant activity of the extracts was determined by DPPH center dot, ABTS(center dot+) and ROO center dot (ORAC) methods. The results indicated that 30% hydroalcoholic fraction (30%E) had the highest total phenolic content (170.68 +/- 3.25 mg GAE/g DW) among the hydroalcoholic extracts and was the most active fraction in antioxidant activity. Besides, intestinal alpha-glucosidase inhibitory activity was conducted to assess the antihyperglycemic ability of the extracts and 30%E showed a significant effect at 10 mg/mL (82.64% inhibition of sucrase and 74.26% inhibition of maltase, respectively). Glucose uptake assay based on HepG2 cells was used to evaluate the glucose uptake effect of 30%E. Five phenolic compounds (tricin 4-O-guaiacylglyceryl ether-7-O-glucopyranoside, genistin, p-coumaric acid, quercetin and genistein) were identified in 30%E using UHPLC-HR-TOFMS. Taken together, the data indicated that sugarcane bagasse can be used as a potential source of bio-active phenolics and may play a role in functional agents exploitation and agro-industrial revenue increase.

• 出版日期2017-7
• 单位华南理工大学; 广州市胸科医院; 南方医科大学