MicroRNA-34a promotes iNOS secretion from pulmonary macrophages in septic suckling rats through activating STAT3 pathway

作者：Cheng, Dong-Liang; Fang, Hong-Xing; Liang, Yuan; Zhao, Yi; Shi, Chang-song^*

来源：Biomedicine & Pharmacotherapy, 2018, 105: 1276-1282.

DOI：10.1016/j.biopha.2018.06.063

• MicroRNA-34a
• iNOS
• Pulmonary macrophages
• Sepsis
• STAT3 pathways

Pediatric sepsis is frequently a fatal condition and a major cause of death globally. The mortality rate of sepsis remains high despite that the advanced therapeutic methods have been carried out. Our research aims to investigate the potentials of miR-34a in the treatment of pediatric sepsis. Results indicated that miR-34a was upregulated in Lipopolysaccharide (LPS)-induced pulmonary macrophages and U937 cell lines. In addition, miR-34a silence reduced the production of iNOS through inactivating STAT3 pathway in U937 cell lines and cecal ligation and puncture (CLP)-induced lung tissues. Besides, high expression of iNOS and STAT3 in cells transfected with miR-34a mimic further validated it. Furthermore, in vivo experiment demonstrated that miR-34a silence protected CLP-induced suckling rats from lung injury. All in all, our study demonstrated that miR-34a promoted iNOS secretion from pulmonary macrophages in LPS-induced sepsis suckling rats through activating STAT3 pathway. These results provided a possibility to convert miR-34a into clinical application.

• 出版日期2018-9
• 单位河南省人民医院; 郑州大学