BREEDING OF Biomphalaria tenagophila IN MASS SCALE

作者：Rosa Florence Mara; Almeida Marques Daisymara P; Maciel Engels; Couto Josiane Maria; Negrao Correa Deborah A; Santana Teles Horacio M; dos Santos Joao Batista; Zech Coelho Paulo Marcos

来源：Revista do Instituto de Medicina Tropical de Sao Paulo, 2013, 55(1): 39-44.

DOI：10.1590/S0036-46652013000100007

• Mass scale breeding
• Biomphalaria
• Biomphalaria tenagophila
• Resistant lineage
• Schistosoma mansoni

An efficient method for breeding Biomphalaria tenagophila (Taim lineage/RS) was developed over a 5-year-period (2005-2010). Special facilities were provided which consisted of four cement tanks (9.4 x 0.6 x 0.22 m), with their bottom covered with a layer of sterilized red earth and calcium carbonate. Standard measures were adopted, as follows: each tank should contain an average of 3000 specimens, and would be provided with a daily ration of 35,000 mg complemented with lettuce. A green-house effect heating system was developed which constituted of movable dark canvas covers, which allowed the temperature to be controlled between 20 - 24 degrees C. This system was essential, especially during the coldest months of the year. Approximately 27,000 specimens with a diameter of 12 mm or more were produced during a 14-month-period. The mortality rates of the newly-hatched and adult snails were 77% and 37%, respectively. The follow-up of the development system related to 310 specimens of B. tenagophila demonstrated that 70-day-old snails reached an average of 17.0 +/- 0.9 mm diameter. The mortality rates and the development performance of B. tenagophila snails can be considered as highly satisfactory, when compared with other results in literature related to works carried out with different species of the genus Biomphalaria, under controlled laboratory conditions.

• 出版日期2013-2