Dose ranging, expanded acute toxicity and safety pharmacology studies for intravenously administered functionalized graphene nanoparticle formulations

作者：Kanakia Shruti; Toussaint Jimmy D; Chowdhury Sayan Mullick; Tembulkar Tanuf; Lee Stephen; Jiang Ya Ping; Lin Richard Z; Shroyer Kenneth R; Moore William; Sitharaman Balaji^*

来源：Biomaterials, 2014, 35(25): 7022-7031.

DOI：10.1016/j.biomaterials.2014.04.066

• Graphene nanoplatelets
• Dextran
• Toxicity
• Dose range
• Biodistribution
• Maximum tolerated dose

Graphene nanoparticle dispersions show immense potential as multifunctional agents for in vivo biomedical applications. Herein, we follow regulatory guidelines for pharmaceuticals that recommend safety pharmacology assessment at least 10 - 100 times higher than the projected therapeutic dose, and present comprehensive single dose response, expanded acute toxicology, toxicokinetics, and respiratory/cardiovascular safety pharmacology results for intravenously administered dextran-coated graphene oxide nanoplatelet (GNP-Dex) formulations to rats at doses between 1 and 500 mg/kg. Our results indicate that the maximum tolerable dose (MTD) of GNP-Dex is between 50 mg/kg <= MTD < 125 mg/kg, blood half-life < 30 min, and majority of nanoparticles excreted within 24 h through feces. Histopathology changes were noted at >= 250 mg/kg in the heart, liver, lung, spleen, and kidney; we found no changes in the brain and no GNP-Dex related effects in the cardiovascular parameters or hematological factors (blood, lipid, and metabolic panels) at doses < 125 mg/kg. The results open avenues for pivotal preclinical single and repeat dose safety studies following good laboratory practices (GLP) as required by regulatory agencies for investigational new drug (IND) application.

• 出版日期2014-8