Nano-functionalization of protein microspheres

作者：Yoon Sungkwon; Nichols William T^*

来源：Applied Surface Science, 2014, 309: 106-111.

DOI：10.1016/j.apsusc.2014.04.194

• Protein microspheres
• Bovine serum albumin
• Ultrasonic synthesis
• Biopolymers
• Surface modification

Protein microspheres are promising building blocks for the assembly of complex functional materials. Here we demonstrate a set of three techniques that add functionality to the surface of protein microspheres. In the first technique, a positive surface charge on the protein spheres is deposited by electrostatic adsorption. Negatively charged silica and gold nanoparticle colloids can then electrostatically bind reversibly to the microsphere surface. In the second technique, nanoparticles are covalently anchored to the protein shell using a simple one-pot process. The strong covalent bond between sulfur groups in cysteine in the protein shell irreversibly binds to the gold nanoparticles. In the third technique, surface morphology of the protein microsphere is tuned through hydrodynamic instability at the water-oil interface. This is accomplished through the degree of solubility of the oil phase in water. Taken together these three techniques form a platform to create nano-functionalized protein microspheres, which can then be used as building blocks for the assembly of more complex macroscopic materials.

• 出版日期2014-8-1