Size-controlled and bio-directed synthesis of ceria nanopowders and their in vitro cytotoxicity effects

作者：Kargar Hadi^*; Ghazavi Hamed; Darroudi Majid

来源：Ceramics International, 2015, 41(3): 4123-4128.

DOI：10.1016/j.ceramint.2014.11.108

• CeO2
• Nanopowders
• Egg white
• Cytotoxicity
• Periodontal fibroblasts cells

The use of bio-based materials such as food-directed products for the synthesis of different nanoparticles (e.g., metal and metal oxide) is of enormous interest to modern nanoscience and nanotechnology. We have developed a simple and green chemistry method with bio-directed, available, and low cost materials for the synthesis of size-controlled ceria nanopowders (CeO2-NPs) using fresh egg white (EW), as an eco-friendly foamy substrate. The proteins of EW, which have various functional groups, played important roles in the synthesis of CeO2-NPs as capping and stabilizing agents. The synthesized CeO2-NPs were characterized by the following title: UV vis spectroscopy, field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric (TGA-DTA) analysis, and powder X-ray diffraction (PXRD). Spherical CeO2-NPs were synthesized at different calcination temperatures (200, 400, 600, 800 degrees C) and FESEM imaging along with its corresponding particles size distribution indicated the formation of nanopowders in size of about 25 nm. The PXRD analysis revealed fluorite cubic structure for CeO2-NPs with preferential orientation at (1 1 1) reflection plane. In vitro cytotoxicity studies on human periodontal fibroblasts cells showed a dose dependent toxicity with non-toxic effect of concentration up to 800 mu g/till. The synthesis of CeO2-NPs in EW was found to be comparable to those obtained from conventional preparation methods that uses hazardous materials proving to be an excellent alternative for the preparation of CeO2-NPs, using food and bio-derived materials.

• 出版日期2015-4