Promotion of the microalgal photo-biocatalytic asymmetric reduction of prochiral ketone by NADPH metabolic regulation

作者：Luo, Wei; Deng, Xin-Xing; Gong, Zhi-Wei; Yang, Zhong-Hua^*

来源：Asia-Pacific Journal of Chemical Engineering, 2016, 11(4): 533-538.

DOI：10.1002/apj.1974

• asymmetric reduction
• biocatalysis
• coenzyme regeneration
• microalgae
• NADPH metabolic regulation

A metabolic regulation method was proposed to increase the microalgal intracellular nicotinamide adenine dinucleotide phosphate (NADPH) by lowering NADPH consumption in the Calvin cycle or accelerating NADPH regeneration in photosynthesis, thereby to promote the asymmetric reduction of prochiral ketone to chiral alcohol catalyzed by microalgae cell. The NADPH consumption was efficiently decreased by inhibiting glyceraldehyde 3-phosphate dehydrogenase with inhibitors, such as iodoacetic acid and Angeli's Salt, which increased the microalgal intracellular NADPH by 80% and 96%, respectively. Correspondingly, the yields of the asymmetric reduction reaction were enhanced by 12.9% and 63.3%, respectively. On the other hand, NADPH regeneration was accelerated by promoting electron transfer and ferredoxin-NADP(+) reductase activity with addition of sodium cholate, sodium acetate and sodium thiosulfate; the intracellular NADPH was increased 73.1%, 60.2% and 70.4%, respectively. The yields of the asymmetric reduction reaction were correspondingly enhanced by 118.4%, 36.7% and 26.2%, respectively. This provides a practical technique to promote microalgae intracellular NADPH amount to enhance asymmetric reduction reaction by photo-biocatalytic processes.

• 出版日期2016-7
• 单位武汉科技大学