Antimicrobial effectiveness of intracanal medicaments on Enterococcus faecalis: chlorhexidine versus octenidine

作者：de Lucena J M V M^*; Decker E M; Walter C; Boeira L S; Loest C; Weiger R

来源：International Endodontic Journal, 2013, 46(1): 53-61.

DOI：10.1111/j.1365-2591.2012.02093.x

• chlorhexidine
• E. faecalis
• intracanal medicaments
• octenidine
• vital staining

de Lucena JMVM, Decker EM, Walter C, Boeira LS, Lost C, Weiger R. Antimicrobial effectiveness of intracanal medicaments on Enterococcus faecalis: chlorhexidine versus octenidine. International Endodontic Journal, 46, 53-61, 2013. Aim To determine the viability of Enterococcus faecalis in infected human root dentine in vitro after exposure to root canal medicaments based on chlorhexidine and octenidine. %26lt;br%26gt;Methodology Human root segments (n = 40) were infected with E. faecalis for 8 weeks. Root dentine samples (rd) collected at week 4 served as individual baseline values. At week 8, the root segments were randomly divided into four test groups (n = 10 each) for the placement of one of the following medicaments in the root canals: calcium hydroxide paste (CH), chlorhexidine gel (CHX-gel) (5.0%), chlorhexidine/gutta-percha points (CHX-GP) (active points (R); Roeko, Langenau, Germany) and octenidine gel (OCT-gel) (5.0%) followed by incubation for 4 weeks. The effect on E. faecalis viability was assessed by two fluorescent dyes (syto 9/propidium iodide) to determine the proportion of viable bacteria (PVB%) and number of colony-forming units (CFU). Mean values and 95% confidence intervals (CI) were calculated for PVB% and log CFU, and the difference between groups was established. %26lt;br%26gt;Results Viable and dead bacterial cells were detected in all rd samples at weeks 4 and 8. The treatment with CHX-gel, CHX-GP and OCT-gel resulted in significantly lower PVB% values with 15.4%, 3.5% and 0%, respectively. No growth (CFU) was recorded for these samples at week 12. When medicated by CH, the PVB% was increased without a corresponding change in CFUs. %26lt;br%26gt;Conclusions In contrast to calcium hydroxide, both CHX and octenidine-based intracanal medicaments were effective in decreasing the viability of E. faecalis. OCT showed the most favourable results and may have potential as an endodontic medicament.

• 出版日期2013-1