Analysis of Disequilibrium Hybridization in Hybrid and Backcross Progenies of Saccharum officinarum x Erianthus arundinaceus

作者:Deng Zu hu; Zhang Mu qing; Lin Wei le; Cheng Fu; Zhang Chui ming; Li Yu chang; Lai Li ping; Lin Yan quan*; Chen Ru kai
来源:Agricultural Sciences in China, 2010, 9(9): 1271-1277.
DOI:10.1016/S1671-2927(09)60216-9

摘要

Erianthus arundinaceus is an important, closely related genus of Saccharum officinarum L. It is therefore important to understand how the chromosomes are transmitted when it hybridizes with sugarcane. The hybrids and backcross progenies of S. officinarum and E. arundinaceus and their parents were used for Karyotype analysis and to study the law of chromosome transmission. The results showed that the somatic chromosome number of both of the E. arundinaceus Hainan92-105 and Hainan92-77 were 2n = 60 = 60sm, belonging to type 1A, and the BC(1) YC01-21 was 2n = 104 = 100m 4sm, belonging to type 2C. The other six tested clones belonged to type 2B. The both F(1)s YC96-66 and YC96-40 that originated from Badila (2n = 80 = 70m 10sm) with E. Arundinaceus were 2n = 70= 68m 2sm, which suggests an n n transmission. The cross between YC96-66 (female parent) and CP84-1198 (male parent, 2n = 120 = 114m 6sm) also followed the same genetic law and the somatic chromosome number of their progeny, YC01-3 (2n = 105 = 95m 10sm). The cross derived from YC96-40 (female) and CP84-1198 (male), YC01-21 had 2n = 104 = 100m 4sm chromosomes, following the same genetic law of n n. However, YC01-36 had 2n = 132 = 130m 2sm chromosomes, which suggests a 2n n chromosome transmission. It can be inferred that the inheritance of chromosomes was very complex in the BC(1). The difference in chromosome number between clones was as high as 28. This could be explained by the 2n n transmission of chromosomes. In addition, as there was not be a regular number of haploids, this phenomenon is termed as disequilibrium hybridization.

  • 出版日期2010-9-20
  • 单位广东省生物工程研究所(广州甘蔗糖业研究所)