Different Allele Prevalence in the Dihydrofolate Reductase and Dihydropteroate Synthase Genes in Plasmodium vivax Populations from China

作者：Miao, Miao; Yang, Zhaoqing; Cui, Long; Ahlum, Jessica; Huang, Yaming; Cui, Liwang^*

来源：The American Journal of Tropical Medicine and Hygiene, 2010, 83(6): 1206-1211.

DOI：10.4269/ajtmh.2010.10-0259

• AMINO-ACID MUTATIONS
• SULFADOXINE-PYRIMETHAMINE
• MALARIA PARASITES
• POINT MUTATIONS
• HENAN PROVINCE
• RESISTANCE
• DHFR
• THAILAND
• DHPS
• ASSOCIATION

Antifolate resistance in Plasmodium vivax is caused by point mutations in genes encoding dihydrofolate reductase (pvdhfr) and dihydropteroate synthase (pvdhps). In this study, we used direct sequencing to survey pvdhfr and pvdhps mutations in 122 clinical P vivax isolates from a central and a southern province of China. For pvdhfr, 36.9% were wild-type, whereas mutations were detected at four codons (57, 58, 61, and 117). The S117N/T mutation was the most prevalent (48.4%), followed by the T61M mutation (18.9%). Six pvdhfr mutant alleles were found, ranging from 37.7% to 0.8%. The dramatically different pvdhfr allele frequencies between the two P vivax populations might be caused by different drug histories or intrinsic difference between temperate and subtropical strains. In contrast, except polymorphisms within a repeat region, no resistance-conferring mutations were detected in pvdhps. Our result suggests that P vivax populations in China may be relatively susceptible to sulfadoxine-pyrimethamine.

• 出版日期2010-12
• 单位昆明医科大学; 广西壮族自治区疾病预防控制中心