Embryo catheter loading and embryo culture techniques: results of a worldwide web-based survey

作者：Christianson Mindy S^*; Zhao Yulian; Shoham Gon; Granot Irit; Safran Anat; Khafagy Ayatallah; Leong Milton; Shoham Zeev

来源：Journal of Assisted Reproduction and Genetics, 2014, 31(8): 1029-1036.

DOI：10.1007/s10815-014-0250-z

• Embryo culture
• Embryo transfer
• In vitro fertilization
• Infertility
• Assisted reproductive techniques

To identify trends in embryo catheter loading and embryo culture techniques performed worldwide.
A retrospective evaluation using the results of a web-based survey, (IVFWorldwide (www.IVF-worldwide.com), was performed.
Responses from 265 centers in 71 countries were obtained. Most centers (97 %) prefered a catheter with its orifice on top, with only 3 % preferring a catheter with the orifice on its side; 41 % preferred a catheter marked for clear ultrasound view. The most commonly-reported methods of embryo loading were medium-air-embryo-airmedium (42 %), medium in catheter with embryo at end (20 %) and medium-air-embryo (15 %). In 68 % of centers the final volume of the catheter was up to 0.3 ml, with only 19 % using 0.3-0.5 ml and 1 % using 0.5-0.7 ml. Using reduced oxygen concentrations for embryo culture was divided between those who used it in combination with the twogas system (34 %) and those who did not use it at all (39 %); 24 % reported using a three-gas system. Most clinics using reduced oxygen concentrations used it throughout the entire culture period. Half of centers (51 %) reported using reduced oxygen concentrations for the entire IVF population while 6 % reserved it only for blastocyst transfer. The use of sequential media was highly dominant with 40 % reporting its use.

• 出版日期2014-8