Differential Expression of Stem Cell Markers and ABCG2 in Recurrent Prostate Cancer

作者：Guzel Esra; Karatas Omer F; Duz Mehmet B; Solak Mustafa; Ittmann Michael; Ozen Mustafa^*

来源：Prostate, 2014, 74(15): 1498-1505.

DOI：10.1002/pros.22867

• prostate cancer
• recurrence
• stem cell markers
• chemoresistance

BACKGROUND. Prostate cancer (PCa) is the second most common tumor type related to mortality in males in the developed countries. Studies have demonstrated that therapeutic tools mostly ineffective to give positive outcome especially for PCa. Cancer stem cells are composed of a small cell population, which are supposed to have roles in tumorigenesis, metastasis, and tumor recurrence after chemo-radiotherapy. The aim of this research is to investigate expressions of stem cell markers in recurrent PCa and non-recurrent PCa tumors as well as in adjacent normal prostate tissues. %26lt;br%26gt;METHODS. We compared the expression of important sternness regulators like SOX2, OCT4, KLF4, and ABCG2 in recurrent, non-recurrent PCa and adjacent normal tissue samples using quantitative real-time polymerase chain reaction (qRT-PCR). %26lt;br%26gt;RESULTS. Our results demonstrated that SOX2 and OCT4 are strongly overexpressed in PCa samples. Recurrent PCa samples are markedly positive for stem cell markers SOX2, OCT4, and KLF4. Furthermore, non-recurrent PCa samples presented low levels of ABCG2, a multidrug resistance protein, compared to both normal and recurrent samples, which might be associated with chemo-sensitivity. %26lt;br%26gt;CONCLUSIONS. Enhanced expression of ABCG2 and stem cell markers including SOX2, OCT4, and KLF4 in the recurrent PCa tissues postulates the suggestion that enrichment for cells with stem cell characteristics in these tissues might be playing a critical role for chemoresistance and recurrence of cancer.

• 出版日期2014-11