科研之友
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摘要
Background BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimens in renal transplant recipients This study develops a sensitive PCR method to detect the viremia and viruna as well as the incidence of BK virus infection in renal transplant recipients
Methods A nested PCR method was developed and a total of 45 paired serum and urine samples from renal transplant recipient patients were collected and tested with the developed assay
Results The threshold of the developed detection assay was 10 copies/mu l of BKV DNA in samples Our results also indicated that about 40% of the urine and 26 7% of serum samples were positive for BKV in renal transplant patients in this study
Conclusion This Nested-PCR method was found a specific sensitive and simple procedure for detection of viruna and viremia of BK virus in renal transplant recipients
- 出版日期2010-11
