ALTERATION IN MORPHOLOGY AND CYTOSKELETON ORGANISATION OF MESENCHYMAL STEM CELLS CULTURED ON PLASMA MODIFIED POLYMER FILMS

作者：Keremidarska Milena^*; Radeva Ekaterina; Pramatarova Lilyana; Krasteva Natalia

来源：Comptes Rendus de l Academie Bulgare des Sciences, 2014, 67(8): 1113-1118.

• mesenchymal stem cells
• plasma modification
• cell morphology

Cell morphology and organisation of actin cytoskeleton are closely related to all essential for cells processes including adhesion, growth and differentiation. Therefore, both cell characteristics can be used to assess the tissue compatibility of a biomaterial. Many approaches have been used to modify biomaterials surface in order to improve their interactions with cells. In the present work, we have modified the surface of a synthetic polymer, plasma polymerised hexamethyldisiloxane (PPHMDS) using two approaches: treatment in ammonia plasma and plasma deposition of detonation nanodiamond (DND) particles, and have studied the alteration in cell morphology, adhesion and actin cytoskeleton organisation in rat mesenchymal stem cells (rMSCs) in order to assess suitability of plasma modified polymer films as cell adhesion substrata for tissue engineering application.

• 出版日期2014