科研之友
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摘要
A new tetrasaccharide derivative, actinotetraose L (1), was isolated from the methanol extract of the mycelia of the grasshopper-associated rare actinobacterium Amycolatopsis sp. HCa1. The structure of the new compound was elucidated by a combination of 1D, 2D NMR (HMQC, HMBC, COSY, and NOESY), and HR-ESI-MS analyses as O-{3,4-di-O-[(E)-2-ethyl-2-butenoyl]- beta-D-glucopyranosyl-(1 -> 2)}-O-{3,4-di-O-[(E)-2-methyl-2-butenoyl]-beta-D-glucopyranosyl-(1 -> 2)}-6,6-di-O-[(E)-2-methyl-2-butenoyl]-alpha-D-glucopyranosyl-(1 -> 1)-alpha-D-glucopyranoside. The immunosuppressive activity and cytotoxicity of the compound were evaluated by T-cell viability and MTT assays. Nonetheless, no significant activity was observed.
