Azilsartan Increases Levels of IL-10, Down-Regulates MMP-2, MMP-9, RANKL/RANK, Cathepsin K and Up-Regulates OPG in an Experimental Periodontitis Model

作者：de Araujo Aurigena Antunes; Varela Hugo; de Castro Brito Gerly Anne; Carvalho Xavier de Medeiros Caroline Addison; Araujo Lorena De Souza; Oliveira do Nascimento Jose Heriberto; De Araujo Junior Raimundo Fernandes

来源：PLos One, 2014, 9(5): e96750.

DOI：10.1371/journal.pone.0096750

Aims: The aim of this study was to evaluate the effects of azilsartan (AZT) on bone loss, inflammation, and the expression of matrix metallo proteinases (MMPs), receptor activator of nuclear factor kappa B ligand (RANKL), receptor activator of nuclear factor kappa B (RANK), osteoprotegerin (OPG), cyclooxygenase-2 (COX-2), and cathepsin K in periodontal tissue in a rat model of ligature-induced periodontitis.
Materials and Methods: Male Wistar albino rats were randomly divided into 5 groups of 10 rats each: (1) nonligated, water; (2) ligated, water; (3) ligated, 1 mg/kg AZT; (4) ligated, 5 mg/kg AZT; and (5) ligated, 10 mg/kg AZT. All groups were treated with saline or AZT for 10 days. Periodontal tissues were analyzed by histopathology and immunohistochemical detection of MMP-2, MMP-9, COX-2, RANKL, RANK, OPG, and cathepsin K. Levels of IL-1 beta, IL-10, TNF-alpha, myeloperoxidase (MPO), and glutathione (GSH) were determined by ELISA.
Results: Treatment with 5 mg/kg AZT resulted in reduced MPO (p<0.05) and IL-1 beta (p<0.05), increased levels of IL-10 (p<0.05), and reduced expression of MMP-2, MMP-9, COX-2, RANK, RANKL, cathepsin K, and increased expression of OPG.
Conclusions: These findings reveal that AZT increases anti-inflammatory cytokines and GSH and decreases bone loss in ligature-induced periodontitis in rats.

• 出版日期2014-5-12