A highly selective and sensitive fluorescent probe for hypochlorous acid and its lysosome-targetable biological applications

作者：Liu, Chang; Jiao, Xiaojie; He, Song; Zhao, Liancheng; Zeng, Xianshun^*

来源：Talanta, 2017, 174: 234-242.

DOI：10.1016/j.talanta.2017.06.012

• Fluorescent probe
• 1,8-Naphthalimide
• Hypochlorous acid
• PET
• Lysosome
• Imaging

A novel lysosome-targetable fluorescence probe PT-1 based on a photoinduced electron transfer (PET) mechanism has been designed and synthesized. For comparison, the probe PT-2 without morpholine moiety as a lysosome-directing group was also synthesized and investigated. Probes PT-1 and PT-2 exhibited high selectivity, high sensitivity (with the detection limits down to the 10(-10) M range) and response in real time (within 10 s) toward HOCl over other reactive oxygen species (ROS). Both PT-1 and PT-2 were cell permeable and enabled them to be used for monitoring of HOCl in living cells. Meanwhile, the probe PT-1 demonstrated an accurately lysosome-targeting ability, and was successfully applied to image of exogenous, endogenous produced HOCl in living cells. The success of subcellular imaging suggested that the probe PT-1 could be used in further applications for the investigation of biological functions and pathological roles of HOCl at organelle levels.

• 出版日期2017-11-1
• 单位哈尔滨工业大学; 天津理工大学