Application of flow cytometry analysis to elucidate the impact of scale-down conditions in Escherichia coli cultivations

作者：Marba Ardebor Anna M^*; Turon Xavier; Neubauer Peter; Junne Stefan

来源：Afinidad, 2016, 73(573): 7-15.

• Scale-down
• flow cytometry
• fed-batch
• heterogeneity
• staining

Inhomogeneities appear in large-scale fed-batch bioprocesses; especially when the distribution of the feeding solution and of the oxygen is uneven. In order to study the consequences of these heterogenic conditions on the culture, scale-down bioreactor experiments have been designed. These studies have revealed that cells exposed to oscillatory conditions are affected on various cellular levels of regulation. However, not many of these studies have been performed on the observation of the behaviour of the single cell level of Escherichia coli. Therefore, flow cytometry (FCM) is chosen as analytical tool to study the cellular viability in the scale-down approaches of a two compartment reactor (Two-CR) and a newly established three compartment reactor (Three-CR). An optimization of different staining methods applied in these experiments is also performed, since staining procedures for flow cytometry studies of bacterial populations are still not well-established yet.

• 出版日期2016-3