Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells

作者：Deng, Wenwen; Fu, Min; Cao, Yue; Cao, Xia; Wang, Miao; Yang, Yan; Qu, Rui; Li, Jun; Xu, Ximing^*; Yu, Jiangnan

来源：Nanomedicine: Nanotechnology, Biology and Medicine , 2013, 9(8): 1181-1191.

DOI：10.1016/j.nano.2013.05.008

• Nanoparticle
• Polysaccharide
• Non-viral vector
• Polyethylenimine
• Mesenchymal stem cells

This study centers on the use of a nanoparticle based on the polysaccharide from Angelica sinensis (ASP) as an efficient and safe non-viral gene vector. After modification with branched low molecular weight polyethylenimine (1200 Da), the cationized ASP (cASP) was combined with the plasmid encoding transforming growth factor-beta 1 (TGF-beta 1) to form a spherical nano-scaled particle (i.e., cASP-pTGF-beta 1 nanoparticle). This nanoparticle was applied to transfect rat bone marrow mesenchymal stem cells and human umbilical cord mesenchymal stem cells. As a result, nanoparticles (cASP/pDNA weight ratio 10: 1) had the greatest transfection efficiency in both cells, which was significantly higher than those of Lipofectamine2000 and PEI (25 kDa). This was in agreement with the findings of the semi-quantitative RT-PCR and live cell imaging. These nanoparticles were also less toxic than Lipofectamine2000 and PEI (25 kDa). Therefore, cASP could be a potential candidate for a novel non-viral gene vector. @@@ From the Clinical Editor: These authors demonstrate the use of a nanoparticle-based efficient and safe non-viral gene vector delivery system via a spherical nanoparticle based on a polysaccharide from Angelica sinensis, with parameters superior to Lipofectamine2000.

• 出版日期2013-11
• 单位江苏大学