Activity of N-acetyl-L-cysteine against biofilm of Staphylococcus aureus and Pseudomonas aeruginosa on orthopedic prosthetic materials

作者：Drago Lorenzo^*; De Vecchi Elena; Mattina Roberto; Romano Carlo L

来源：The International Journal of Artificial Organs, 2013, 36(1): 39-46.

DOI：10.5301/ijao.5000135

• Prosthetic joint infections
• N-acetyl-L-cysteine
• Staphylococcus aureus
• Pseudomonas aeruginosa
• Bacterial biofilm

Purpose: Staphylococcus aureus and Pseudomonas aeruginosa are frequently isolated from biofilm-associated orthopedic implant infections. This study assessed the activity of N-acetyl-L-cysteine at inhibitory and subinhibitory concentrations against biofilm formed by S. aureus and P. aeruginosa on prosthetic materials. %26lt;br%26gt;Methods: S. aureus and P. aeruginosa biofilm on smooth polyethylene and sand-blasted titanium discs was determined by means of a spectrophotometric assay before and at 3, 6, and 18 hours after addition of N-acetyl-L-cysteine. %26lt;br%26gt;Results: N-acetyl-L-cysteine was able to disaggregate biofilm produced by S. aureus and P. aeruginosa on polyethylene and titanium discs after 3 h of incubation. A different rate in eradication of biofilm produced by P. aeruginosa was seen between polyethylene and titanium (50% vs. 20% respectively vs. biofilm formed before treatment). Results were confirmed by confocal laser scanning microscopy. %26lt;br%26gt;Conclusions: The results suggest a possible role of N-acetyl-L-cysteine in the treatment of orthopedic prosthetic infections.

• 出版日期2013-1