Anatomy of plasmid DNAs with anti-silencing elements

作者：Kanda Genki N; Miyamoto Shiho; Kobayashi Miwako; Matsuoka Ichiro; Harashima Hideyoshi; Kamiya Hiroyuki^*

来源：International Journal of Pharmaceutics, 2014, 464(1-2): 27-33.

DOI：10.1016/j.ijpharm.2014.01.025

• Transgene expression
• Nonviral vector
• Anti-silencing element
• Hydrodynamics-based method

The transience of transgene expression is a major obstacle in the development of nonviral vectors. The CpG-free and pLIVE plasmids reportedly achieve long-term transgene expression in mouse liver. In this work, the anti-silencing elements within these plasmids were studied. The effects of plasmid that was being silenced on transgene expression from the CpG-free plasmid and those of transgene expression at early time points on silencing were also examined. The results suggested that the backbone sequence of the CpG-free plasmid and the 3%26apos; untranslated region of the albumin gene of the pLIVE plasmid contribute to durable expression. In addition, no influence of the silencing of another plasmid on the duration of CpG-free plasmid expression or of transgene expression at early time points on silencing was detected.

• 出版日期2014-4-10