Altered proportion of CCR2(+) and CX3CR1(+) circulating monocytes in neovascular age-related macular degeneration and polypoidal choroidal vasculopathy

作者：Subhi Yousif^*; Nielsen Marie Krogh; Molbech Christopher R; Sorensen Torben L

来源：Clinical and Experimental Ophthalmology, 2018, 46(6): 661-669.

DOI：10.1111/ceo.13152

• choroidal neovascularization
• monocytes
• chemokine receptors
• wet macular degeneration

BackgroundWe investigated the expression of chemokine receptors CCR2 (C-C chemokine receptor) 2 and CX3CR1 (C-X3-C receptor 1) on circulating monocyte subsets in patients with neovascular age-related macular degeneration (AMD) and patients with polypoidal choroidal vasculopathy (PCV).
MethodsWe recruited patients with neovascular AMD, patients with PCV and age-matched healthy controls for this prospective case-control study. All participants underwent comprehensive clinical examination and imaging. Freshly sampled venous blood was prepared for flow cytometry, where we determined the proportion of CCR2(+)- and CX3CR1(+)-positive cells in monocyte subsets identified using monocyte identification and subgrouping surface markers CD14, CD16 and HLA-DR.
ResultsPatients with neovascular AMD had significantly increased proportion of CCR2(+) and CX3CR1(+) non-classical monocytes. PCV type 1 was associated with significantly increased CCR2(+) and CX3CR1(+) in all monocyte subsets when compared to PCV type 2.
ConclusionsNeovascular AMD is associated with increased expression of angiogenesis-associated chemokine receptors in the pro-inflammatory non-classical monocytes. PCV differs from neovascular AMD immunologically and show immunological heterogeneity across angiographic subtypes.

• 出版日期2018-8