Analysis of genotoxic damage induced by dacarbazine: an in vitro study

作者：Khan Fauzia^*; Sherwani Asma Farhat; Afzal Mohammad

来源：Toxin Reviews, 2010, 29(3-4): 130-136.

DOI：10.3109/15569543.2010.516463

• lymphocyte culture
• anticancer drugs
• Chromosomal aberrations
• cytochalasin-B
• micronucleus
• nuclear division index

Dacarbazine is one of the alkylating anticancer drugs being routinely used for the treatment of a number of neoplasias. Besides targeting cancer cells, these anticancer drugs also interact with genetic material of normal cells. In the present study, in vitro genotoxic effects of dacarbazine have been assessed employing cytogenetic assays. A variety of chromosomal aberrations were scored and significant increase in micronucleus and chromosomal aberration frequency was observed. Nuclear division index showed cell cycle delay towards higher concentrations. A number of necrotic and apoptotic cells were also observed at higher concentrations. Dacarbazine was found to be a potent inducer of genotoxic damage in human lymphocytes in vitro.

• 出版日期2010-8