Aminotriazole attenuated carbon tetrachloride-induced oxidative liver injury in mice

作者：Deng, Xinyu; Wu, Kunwei; Wan, Jingyuan; Li, Longjiang; Jiang, Rong; Jia, Mengying; Jing, Yuping; Zhang, Li^*

来源：Food and Chemical Toxicology, 2012, 50(9): 3073-3078.

DOI：10.1016/j.fct.2012.05.052

• Aminotriazole
• Catalase
• Carbon tetrachloride
• Oxidative stress
• Acute liver injury

Carbon tetrachloride (CCl4) has been used extensively to study xenobiotic-induced oxidative liver injury. Catalase (CAT) is a major antioxidant enzyme while aminotriazole (ATZ) is commonly used as a CAT inhibitor. In the present study, the effects of ATZ on CCl4-induced liver injury were investigated. Our experimental data showed that pretreatment with ATZ significantly decreased CCl4-induced elevation of serum aspartate transaminase (AST) and alanine transaminase (ALT) and improved hepatic histopathological abnormality. ATZ dose-dependently inhibited the activity of CAT, but it reduced the content of H2O2 and the levels of malondialdehyde (MDA) in liver tissues. ATZ decreased plasma level of pro-inflammatory cytokines (TNF-alpha. and IL-6) and reduced hepatic levels of myeloperoxidase (MPO). In addition, posttreatment with ATZ also decreased the level of ALT and AST. These data indicated that ATZ effectively alleviated CCl4-induced oxidative liver damage. These findings suggested that ATZ might have potential value in preventing oxidative liver injury.

• 出版日期2012-9
• 单位重庆医科大学