Association between Genetic Polymorphisms of DNA Repair Genes and Chromosomal Damage for 1,3-Butadiene-Exposed Workers in a Matched Study in China

作者：Xiang Menglong; Sun Lei; Dong Xiaomei; Yang Huan; Liu Wen bin; Zhou Niya; Han Xue; Zhou Ziyuan; Cui Zhihong; Liu Jing yi; Cao Jia; Ao Lin^*

来源：Biomed Research International, 2015, 2015: 234675.

DOI：10.1155/2015/234675

The aim of the study was to examine the association between polymorphisms of DNA repair genes and chromosomal damage of 1,3-butadiene-(BD-) exposed workers. The study was conducted in 45 pairs of occupationally exposed workers in a BD product workshop and matched control workers in an administrative office and a circulatory water workshop in China. Newly developed biomarkers (micronuclei, MNi; nucleoplasmic bridges, NPBs; nuclear buds, NBUDs) in the cytokinesis-blocked micronucleus (CBMN) cytome assay were adopted to detect chromosomal damage. PCR and PCR-restriction fragment length polymorphism (RFLP) are adopted to analyze polymorphisms of DNA repair genes, such as X-ray repair cross-complementing Group 1 (XRCC1), O-6 -methylguanine-DNA methyltransferase (MGMT), poly (adenosine diphosphate-ribose) polymerases (ADPRT), and apurinic/apyrimidinic endonucleases (APE1). The BD-exposed workers exhibited increased frequencies of MNi and NPBs when compared to subjects in the control group. The results also show that the BD-exposed workers carrying XRCC1 diplotypes TCGA-CCGG (4.25 +/- 2.06%) (FR = 2.10, 95% CI: 1.03-4.28) and TCGG-TCGA (5.80 +/- 3.56%) (FR = 2.75, 95% CI: 0.76-2.65) had statistically higher NBUD frequencies than those who carried diplotype TCGG-TCGG (1.89 +/- 1.27%). Our study suggests that polymorphisms of XRCC1 gene may influence chromosomal damage in BD-exposed workers.

• 出版日期2015
• 单位中国人民解放军陆军军医大学