GROWTH, FATTY ACID PROFILE IN MAJOR LIPID CLASSES AND LIPID FLUIDITY OF AURANTIOCHYTRIUM MANGROVEI SK-02 AS A FUNCTION OF GROWTH TEMPERATURE

作者：Chodchoey Kanokwan^*; Verduyn Cornelis

来源：Brazilian Journal of Microbiology, 2012, 43(1): 187-200.

• Anisotropy measurement
• Aurantiochytrium sp.
• Docosahexaenoic acid
• lipid class composition
• membrane fluidity

Aurantiochytrium mangrovei Sk-02 was grown in a medium containing glucose (40 g/l), yeast extract (10 g/L) and sea salts (15 g/L) at temperatures ranging from 12 to 35 degrees C. The fastest growth (mu(max)=0.15 h(-1)) and highest fatty acid content of 415 mg/g-dry cell weight were found in the cells grown at 30 degrees C. However, the cells grown at 12 degrees C showed the highest percentage of polyunsaturated fatty acid (PUFA) (48.6% of total fatty acid). The percentage of docosahexaenoic acid (DHA) and pentadecanoic acid (C15:0) decreased with an increase in the growth temperature, whereas, palmitic acid (C16:0), stearic acid (C18:0) and DPA (C22:5n6) increased with an increase in the growth temperature. The composition of the major lipid class (%w/w) was slightly affected by the growth temperature. The fluidity of the organelle membrane or intracellular lipid (by DPH measurement) decreased with an increase in the growth temperatures, while the plasma membrane fluidity (by TMA-DPH measurement) could still maintain its fluidity in a wide range of temperatures (15 - 37 degrees C). Furthermore, the distribution of DHA was found to be higher (36 - 54%) in phospholipid (PL) as compared to neutral lipid (NL) (20 - 41%).

• 出版日期2012-3