What sample preparation should be chosen for targeted MS monoclonal antibody quantification in human serum?

作者：Vialaret Jerome; Broutin Sophie; Pugnier Celia; Santele Sophie; Jaffuel Aurore; Barnes Alan; Tiers Laurent; Pelletier Laurent; Lehmann Sylvain; Paci Angelo; Hirtz Christophe^*

来源：Bioanalysis, 2018, 10(10): 723-735.

DOI：10.4155/bio-2018-0027

• automation
• bevacizumab
• MS
• nSMOL
• protein-A purification
• therapeutic drug monitoring

Aim: Monoclonal antibody-based treatment of cancer has been established as one of the most successful therapeutic strategies. Materials & methods: In this work, we developed a workflow based on an automated protein-A capture and LC-MS/MS analysis to quantify bevacizumab on patient serum during treatment. This analytical approach was fully validated and compared with a commercially available Monoclonal antibody-based treatment preparation (nanosurface and molecular-orientation limited kit). Results: The analytical comparison of the two preparative workflows based on protein-A capture gave similar results with a better lower limit of quantification for the nanosurface and molecular-orientation limited kit (0.26986 vs 1.9565 mu g/ml). Conclusion: LC-MS/MS has clear advantages compared with ELISA when considering method development time, multiplexing capacities and absolute quantification with internal standardization.

• 出版日期2018-5