Differential Requirement for the IKK beta/NF-kappa B Signaling Module in Regulating TLR- versus RLR-Induced Type 1 IFN Expression in Dendritic Cells

作者：Wang Xingyu; Wang Junmei; Zheng Hong; Xie Mengyu; Hopewell Emily L; Albrecht Randy A; Nogusa Shoko; Garcia Sastre Adolfo; Balachandran Siddharth; Beg Amer A^*

来源：The Journal of Immunology, 2014, 193(5): 2538-2545.

DOI：10.4049/jimmunol.1400675

Host innate-immune responses are tailored by cell type to control and eradicate specific infectious agents. For example, an acute RNA virus infection can result in high-level expression of type 1 IFNs by both conventional dendritic cells (cDCs) and plasmacytoid dendritic cells (pDCs), but whereas cDCs preferentially use RIG-I like receptor (RLR) signaling to produce type 1 IFNs, pDCs predominantly use TLRs to induce these cytokines. We previously found that the I kappa B kinase beta (IKK beta)/NF-kappa B pathway regulates early IFN-beta expression, but not the magnitude of type 1 IFN expression following RLR engagement. In this study, we use IKK beta inhibition and mice deficient in IKK beta or canonical NF-kappa B subunits (p50, RelA/p65, and cRel) to demonstrate that the IKK beta/NF-kappa B axis is critical for virus-induced type 1 IFN expression in pDCs, but not in cDCs. We also reveal a crucial and more general requirement for IKK beta/NF-kappa B in TLR- but not RLR-induced expression of type 1 IFNs and inflammatory cytokines. Together, these findings reveal a previously unappreciated specificity of the IKK beta/NF-kappa B signaling axis in regulation of antimicrobial responses by different classes of pattern recognition receptors, and therefore by individual cell types reliant on particular pattern recognition receptors for their innate-immune transcriptional responses.

• 出版日期2014-9-1