Absolute quantification by droplet digital PCR versus analog real-time PCR

作者：Hindson, Christopher M.; Chevillet, John R.; Briggs, Hilary A.; Gallichotte, Emily N.; Ruf, Ingrid K.; Hindson, Benjamin J.; Vessella, Robert L.; Tewari, Muneesh^*

来源：Nature Methods, 2013, 10(10): 1003-+.

DOI：10.1038/nmeth.2633

• DNA COPY NUMBER
• MICRORNAS
• QUANTITATION
• CANCER
• SERUM
• RNA

Nanoliter-sized droplet technology paired with digital PCR (ddPCR) holds promise for highly precise, absolute nucleic acid quantification. Our comparison of microRNA quantification by ddPCR and real-time PCRCR revealed greater precision (coefficients of variation decreased 37-86%) and improved day-to-day reproducibility (by a factor of seven) of ddPCR but with comparable sensitivity. When we applied ddPCR to serum microRNA biomarker analysis, this translated to superior diagnostic performance for identifying individuals with cancer.

• 出版日期2013-10