A versatile strategy for gene trapping and trap conversion in emerging model organisms

作者：Kontarakis Zacharias; Pavlopoulos Anastasios; Kiupakis Alexandros; Konstantinides Nikolaos; Douris Vassilis; Averof Michalis^*

来源：Development, 2011, 138(12): 2625-2630.

DOI：10.1242/dev.066324

• Parhyale
• Gene trapping
• phi C31 integrase
• Regeneration
• Transgenesis
• iTRAC

Genetic model organisms such as Drosophila, C. elegans and the mouse provide formidable tools for studying mechanisms of development, physiology and behaviour. Established models alone, however, allow us to survey only a tiny fraction of the morphological and functional diversity present in the animal kingdom. Here, we present iTRAC, a versatile gene-trapping approach that combines the implementation of unbiased genetic screens with the generation of sophisticated genetic tools both in established and emerging model organisms. The approach utilises an exon-trapping transposon vector that carries an integrase docking site, allowing the targeted integration of new constructs into trapped loci. We provide proof of principle for iTRAC in the emerging model crustacean Parhyale hawaiensis: we generate traps that allow specific developmental and physiological processes to be visualised in unparalleled detail, we show that trapped genes can be easily cloned from an unsequenced genome, and we demonstrate targeting of new constructs into a trapped locus. Using this approach, gene traps can serve as platforms for generating diverse reporters, drivers for tissue-specific expression, gene knockdown and other genetic tools not yet imagined.

• 出版日期2011-6-15