A specific molecular beacon probe for the detection of human prostate cancer cells

作者：Jiang Yu Lin^*; McGoldrick Christopher A; Yin Deling; Zhao Jing; Patel Vini; Brannon Marianne F; Lightner Janet W; Krishnan Koyamangalath; Stone William L

来源：Bioorganic & Medicinal Chemistry Letters, 2012, 22(11): 3632-3638.

DOI：10.1016/j.bmcl.2012.04.055

• Molecular beacon
• Fluorescent probe
• Fluorogenic
• Chromogenic
• Detection
• Prostate cancer
• Carboxylesterase activity
• Biomarker
• Porcine liver esterase

The small-molecule, water-soluble molecular beacon probe 1 is hydrolyzed by the lysate and living cells of human prostate cancer cell lines (LNCaP), resulting in strong green fluorescence. In contrast, probe 1 does not undergo significant hydrolysis in either the lysate or living cells of human nontumorigenic prostate cells (RWPE-1). These results, corroborated by UV-Vis spectroscopy and fluorescent microscopy, reveal that probe 1 is a sensitive and specific fluorogenic and chromogenic sensor for the detection of human prostate cancer cells among nontumorigenic prostate cells and that carboxylesterase activity is a specific biomarker for human prostate cancer cells.

• 出版日期2012-6-1