Use of reversed phase high pressure liquid cromatography for the physicochemical and thermodynamic characterization of oxyresveratrol/beta-cyclodextrin complexes

作者：Rodriguez Bonilla Pilar; Manuel Lopez Nicolas Jose; Garcia Carmona Francisco

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2010, 878(19): 1569-1575.

DOI：10.1016/j.jchromb.2010.04.016

• Oxyresveratrol
• Stilbene
• Cyclodextrins
• Nanoencapsulation
• Reversed-phase liquid chromatography

Knowledge of the complexation process of oxyresveratrol with beta-cyclodextrin (beta-CD) under different physicochemical conditions is essential if this potent antioxidant compound is to be used successfully in both food and pharmaceutical industries as ingredient of functional foods or nutraceuticals, despite its poor stability and bioavailability In this paper, the complexation of oxyresveratrol with natural CDs was investigated for first time using RP-HPLC and mobile phases to which alpha-, beta-, and gamma-CD were added Among natural CDs, the interaction of oxyresveratrol with beta-CD was mote efficient than with alpha- and gamma-CD The decrease in the retention times with increasing concentrations of beta-CD (0-4 mM) showed that the formation constants (K(F)) of the oxyresveratrol/beta-CD complexes were strongly dependent on both the water-methanol proportion and the temperature of the mobile phase employed However, oxyresveratrol formed complexes with beta-CD with a 1 1 stoichiometry in all the physicochemical conditions tested Moreover, to obtain information about the mechanism of the oxyresveratiol affinity for beta-CD. the thermodynamic parameters Delta G degrees, Delta H degrees and Delta S degrees were obtained Finally, to gain information on the effect of the structure of different compounds belonging to the stilbenoids family on the K(F) values, the complexation of other molecules, resveratrol, pterostilbene and pinosylvin.

• 出版日期2010-6-1