Dietary fish oil is antihypertrophic but does not enhance postischemic myocardial function in female mice

作者：Huggins Catherine E; Curl Claire L; Patel Ruchi; McLennan Peter L; Theiss Mandy L; Pedrazzini Thierry; Pepe Salvatore; Delbridge Lea M D^*

来源：American Journal of Physiology - Heart and Circulatory Physiology, 2009, 296(4): H957-H966.

DOI：10.1152/ajpheart.01151.2008

• polyunsaturated fatty acids
• ischemia-reperfusion
• Langendorff perfused hearts

Huggins CE, Curl CL, Patel R, McLennan PL, Theiss ML, Pedrazzini T, Pepe S, Delbridge LM. Dietary fish oil is antihypertrophic but does not enhance postischemic myocardial function in female mice. Am J Physiol Heart Circ Physiol 296: H957-H966, 2009. First published January 30, 2009; doi:10.1152/ajpheart.01151.2008.-Clinically and experimentally, a case for omega-3 polyunsaturated fatty acid (PUFA) cardioprotection in females has not been clearly established. The goal of this study was to investigate whether dietary omega-3 PUFA supplementation could provide ischemic protection in female mice with an underlying genetic predisposition to cardiac hypertrophy. Mature female transgenic mice (TG) with cardiac-specific overexpression of angiotensinogen that develop normotensive cardiac hypertrophy and littermate wild-type (WT) mice were fed a fish oil-derived diet (FO) or PUFA-matched control diet (CTR) for 4 wk. Myocardial membrane lipids, ex vivo cardiac performance (intraventricular balloon) after global no-flow ischemia and reperfusion (15/30 min), and reperfusion arrhythmia incidence were assessed. FO diet suppressed cardiac growth by 5% and 10% in WT and TG, respectively (P < 0.001). The extent of mechanical recovery [rate-pressure product (RPP) = beats/min x mmHg] of FO-fed WT and TG hearts was similar (50 +/- 7% vs. 45 +/- 12%, 30 min reperfusion), and this was not significantly different from CTR-fed WT or TG. To evaluate whether systemic estrogen was masking a protective effect of the FO diet, the responses of ovariectomized (OVX) WT and TG mice to FO dietary intervention were assessed. The extent of mechanical recovery of FO-fed OVX WT and TG (RPP, 50 +/- 4% vs. 64 +/- 8%) was not enhanced compared with CTR-fed mice (RPP, 60 +/- 11% vs. 80 +/- 8%, P = 0.335). Dietary FO did not suppress the incidence of reperfusion arrhythmias in WT or TG hearts (ovary-intact mice or OVX). Our findings indicate a lack of cardioprotective effect of dietary FO in females, determined by assessment of mechanical and arrhythmic activity postischemia in a murine ex vivo heart model.

• 出版日期2009-4